Logo de 22nd International Symposium on Bioluminescence and Chemiluminescence & 20th International Symposium on Luminescence Spectroscopy
Wait a moment for the page to load

Enter your credentials

Fechar janela

Sign up

Forgot your password?

Logo 22nd ISBC & 20th ISLS
MENU

Abstracts

Luminescent materials for imaging, sensors and theranostics

Development of Coelenterazine Analogues for High-Brightness Multicolor Bioluminescence

Genta Kamiy1, Nobuo Kitada1, Furuta Tadaomi2, Takashi Hirano1, S. Kim3, Shojiro Maki1

1Department of Engineering Science, The University of Electro-Communications, 2School of Life Science and Technology, Tokyo Institute of Technology, 3Environmental Management Research Institute, Advanced Industrial Science and Technology

E-mail: kamiya0801@uec.ac.jp

Marine bioluminescence emits light through an enzymatic reaction where the enzyme (luciferase) catalyzes the oxidation of the bioluminescent substrate, coelenterazine (CTZ). Due to the absence of drawbacks such as excitation light-induced interference, phototoxicity, photobleaching, and high background, it enables highly sensitive and non-invasive imaging. Furthermore, various applications in bioassays and bioimaging have been reported by artificially modifying and optimizing luciferases and CTZ. Our research group has successfully developed artificial marine bioluminescence systems such as bioluminescent probes that emit light upon stimulation by specific ligands and multicolor bioluminescence systems using CTZ analogues. However, these artificial bioluminescence systems have several issues. It has been observed that the bioluminescence intensity significantly decreases in multicolor bioluminescence systems that cover a range from blue to red (400-650 nm). Therefore, in this study, we focused on exploring chemical structures that exhibit high brightness and multicolor bioluminescence.We have designed and synthesized new CTZ analogues. We performed bioluminescence evaluations of these CTZ analogues with various marine luciferases. The characterization revealed that CTZ analogues luminesce with blue to orange-colored bioluminescence spectra with marine luciferase. Furthermore, they demonstrated high bioluminescence intensity with various marine luciferases, leading to the successful development of a high-brightness and multicolor bioluminescence system.

Keywords: Marine Bioluminescence, Coelenterazine, Multicolor

Acknowledgments: This work was supported by grants from the Japan Society for the Promotion of Science (JSPS grants 21H04948, 20K21851, and 17H01215) and JST SPRING (Grant Number JPMJSP2131).


Search for submitted abstracts

You can select one or more search filters