Abstracts

Biological imaging across scales can reveal dynamics of complex processes such as cancer metastasis, immune function, host-microbiome interation, connections of neurons and leads to a better understanding of physiology. Imaging modalities are applied as separate niches in biomedical research, where optical microscopy and mesoscopy focus on subcellular, cellular and tissue slice imaging and nuclear and MR imaging are applied to living subject. Bioluminescence imaging which can generate bright signals and high signal to noise ratios can nowadays be applied for microscopy, mesoscopy and mascroscopy imaging.This lecture will discuss recent developments from my laboratory on bioluminescence microscopy imaging of single cells and engineered tissues on chips, mesoscopic imaging in intact ex vivo tissues and organs and on multiplexed bioluminescence imaging in small and large animals to elucidate cancer-immune cells and host-microbiome interactions. In particular, cancer cell based sensors based on multicolor bioluminescent readouts and how novel unmixing algorithms and detectors can improve resolution of multiplex bioluminescent signals will be highlighted.