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Logo 22nd ISBC & 20th ISLS


Analytical, clinical and medical applications of luminescence

Novel Luciferins synthesis and their applications for pesticide detection

Pratchaya Watthaisong1, Ittiphat Klayparn1, Philaiwarong Kamutira2, Chadaporn Kantiwiriyawanitch1, Ruchanok Tinikul2, Yoshihiro Ohmiya3, Pimchai Chaiyen1

1School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand, 2Department of Biochemistry and Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok, Thailand, 3National Institute of Advanced Industrial Science and Technology (AIST), Osaka, Japan

E-mail: pratchaya.w_s17@vistec.ac.th

Pesticide contamination in food and environment is a major problem in Thailand and many countries around the world because it can cause long-term negative effects on human health. We have developed an enzymatic cascade reaction to degrade various pesticides and change them into firefly luciferin derivatives (~51% yield) which are widely used in detection applications. We have shown that the bioluminescence of novel luciferins is red-shifted with maximum emission at 663 nm (5’,7’-dimethoxy luciferin, 5’,7’-DiOMeLH2). Reactions of 5’-methyl luciferin (5’-MeLH2) and 5’,7’-dimethyl luciferin (5’,7’-DiMeLH2) with various beetle luciferases provide higher light intensity than that of natural D-luciferin. Remarkably, the novel 5’,7’-DiOMeLH2 and 5’,7’-DiMeLH2 give steadily light emission with a slower rate of light decay (3.2-fold) than D-luciferin. These novel luciferins thus can provide substantially stronger bioluminescence that the natural D-luciferin, making them useful and suitable for various applications including real-time bioluminescence imaging. Moreover, we have developed the enzymatic reaction to detect pesticide contamination in food, environmental, and biological samples called Luminescence-related Method for Specific detection (LUMOS). LUMOS technology provides high sensitivity of detection in a range of ppt levels with the accuracy comparable to the gold standard methods of using HPLC-MS. We have used LUMOS technology in local communities in northern Thailand as a surveillance tool to detect pesticide contamination in food and environmental samples.

Keywords: bioluminescence, luciferin, luciferase, pesticide, environment, analytical

Acknowledgments: The research was supported by The Thailand Science Research and Innovation (TSRI) grant, Program Management Unit B (PMU-B) and VISTEC

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